IRIANTI, Netersta Yunitarela (2019) Isolasi Dan Identifikasi Senyawa Aktif Dari Ekstrak Etanol Daun Wulukula (Lagenaria Siceraria (Molina) Standly) Asal Desa Ulupulu Kabupaten Nagekeo Dan Uji Aktivitas Antioksidan Dengan Metode DPPH. Diploma thesis, Universitas Katolik Widya Mandira.
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Abstract
The purpose of this study was to determine the active compounds identified, the percent inhibitory activity of DPPH and IC50 values from the ethanol extract of Wulukula leaves (lagenaria siceraria (Molina) Standly) from Ulupulu Village, Nagekeo Regency. This research was conducted using extraction methods, chromatography, UV-Vis and IR spectrophotometry and DPPH methods. Wulukuladari dry powder extraction from 250 grams obtained by ethanol extract as much as 20.81 grams with a yield of 8.324% w / w. Phytochemical test results showed positive containing flavonoid compounds, alkaloids, and saponins. Identification of isolates in the ethanol fraction using UV-Vis spectrophotometer showed absorption at a maximum wavelength of 285 nm (fraction A), 295 nm (fraction B), 310 nm (band I) and 280 nm (band II) on (fraction C), and 305 nm (band I) and 295 nm (band II) on (fraction D). While the results of IR spectrum on (fractions A, B, C, and D) showed the existence of stretching vibrations of OH phenol groups (3500-3000 cm -1 ), C=C aromatics (1500-1400 cm-1 ), C-C aromatics (1400-1300 cm-1 ), C-O ether (1300-1000 cm-1 ), C-H aromatic stretching vibrations (1300-1000 cm-1 ) C-H aromatic bends (1000-650 cm-1 ), C-H aliphatic stretches (2950-2800 cm-1 ), and C=O carbonyl (1870-1540 cm-1 ).. From the results of UV-Vis and IR spectrophotometer analysis, it was concluded that the isolate contained flavonoid compounds suspected to be flavonone group. The inhibitory activity of DPPH by sample extracts was 33.86% (25 ppm), 39.65% (50 ppm), 49.01% (100 ppm), 65.87% (125 ppm), and 81.55% (250 ppm). And has an IC50 value of 96.82 ppm.
| Item Type: | Thesis (Diploma) |
|---|---|
| Uncontrolled Keywords: | (Lagenaria Siceraria (Molina) Stanley) phytochemical, Antioxidant |
| Subjects: | Q Science > QD Chemistry Q Science > QK Botany S Agriculture > SB Plant culture |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Program Studi Kimia |
| Depositing User: | S.Kom Sela Mikado |
| Date Deposited: | 24 Aug 2020 05:25 |
| Last Modified: | 24 Aug 2020 05:25 |
| URI: | http://repository.unwira.ac.id/id/eprint/3001 |
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