Isolasi Dan Karakterisasi Bakteri Tanah Pada Lahan Pertanian Kacang Tanah (Arachis Hypogaea) Di Desa Oematnunu Kecamatan Kupang Barat

SAIT, Yohana Bengan (2020) Isolasi Dan Karakterisasi Bakteri Tanah Pada Lahan Pertanian Kacang Tanah (Arachis Hypogaea) Di Desa Oematnunu Kecamatan Kupang Barat. Diploma thesis, Unika Widya Mandira.

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Abstract

This study is entiled “Isolation and Characterization of soil Bacteria in Oematnunu village, West Kupang District, Kupang Regency”.The formulation of the problem in this study is “are there any soil bacterial isolates, specifically rhizobium in peanut farm in oeli’I village wast kupang district, kupang regency”. This study aims to ensure the presence or absence of soil bacterial isolates and absence of soil bacterial isolates and the characterization of rhisobium in peanut farms in Oematnunu village, west kupang distict kupang regency.This study uses a qualitative descriptive method. The sample in this study consists of the soil and litter taken in peanut farming in Oematnunu village, west kupang district, kupang regency. Bacterial isolation in this study was done using MSM (Minium Salt Medium) media the use of this media aims to obtain the desired soil bacterial isolates. The obtained bacterial isolates were grown on selective media YEMA (Yeast Manitol Agar Extract), then added Congo Red (CR) and Brom Thimol Blue (BTB) and then obtained to isolates of rhizobium bacteria then the characteristics of rhizobium were indentited using bacterial characterization patters as described in the book Bergeys’s Manual Of Determinative Bacteria 9th, and obtained 5 isolates, belonging to the rhizobium bacteria.

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