Identifikasi Senyawa Aktif Ekstrak Batang Kara Benguk (Mucuna Pruriens, L.) Asal Desa Teun, Kabupaten Belu

AKUN, Antonius (2020) Identifikasi Senyawa Aktif Ekstrak Batang Kara Benguk (Mucuna Pruriens, L.) Asal Desa Teun, Kabupaten Belu. Undergraduate thesis, Universitas Katolik Widya Mandira.

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Abstract

Research has been carried out on the identification of the active compound extract of velvet bean stem (Mucuna pruriens L) from Teun Village, Belu Regency. The purpose of this study was to determine the content of active compounds contained in the stem extract of Mucuna pruriens. The method used is the maceration to extract active compounds, identification of compound groups using phytochemical methods, fractionation by chromatography and structural determination by UV-Vis and Infrared spectrophotometry. Identification of this compound begins with extracting 100 gr of simplinsia dissolved with acetone. The resulting filtrate, then extracted with 30% ethanol and concentrated using a rotary evaporator. Research result; obtained extract yield of 46.17%. The sample extract is blackish brown, in the form of a paste and sticky. Phytochemical analysis obtained alkaloid compounds, saponins, tannins, proteins and amino acids. Fractionation using eluent combination of n-butanol: acetic acid: water (4:1:1 v/v), there were 5 fractions that had a single spot Rf value of 0.3, then collected and concentrated. Identification of fractions using a UV-Vis spectrophotometer is absorbed at a maximum wavelength of 295 nm with an absorption value of 4.088. whereas the results of structural elucidation using an infrared spectrophotometer, there were several functional groups O-H alcohol, C-H aliphatic, C=C aromatic, C-H aromatic. This functional groups constitutes the structure of the active compounds contained in the stem extract of Mucuna pruriens.

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