POTENCY OF METHANOL EXTRACT OF ARTOCARPUS CHAMPEDEN STEM BARK TO INHIBITS THE GROWTH OF PLASMODIUM FALCIPARUM

TAEK, Maximus M. and WIDYAWARUYANTI, Aty and NINDATU, Maria (2012) POTENCY OF METHANOL EXTRACT OF ARTOCARPUS CHAMPEDEN STEM BARK TO INHIBITS THE GROWTH OF PLASMODIUM FALCIPARUM. In: PROCEEDINGS OF INTERNATIONAL CONFERENCE ON MEDICINAL PLANTS: scientification of jamu (evidence based jamu development): a breakthrough program from plant to medicine for health care, 11-13 oktober 2012, Purwekerto.

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Abstract

Methanol exctract obtained from a three-steps successively exctraction of the Cempedak (Artocarpus champeden) stem bark was examined for its antimalarial activity. The exctration was done firstly with n-hexane , secondly with dichloromethane and then with methanol. The methanol exctract then subjected to examination with the chloroquine-sensitive and chloroquine-resistant strains of Plasmodium Falciparum according to an in-vitro testing procedure developed by Trager and Jensen. The examination was conducted with non-synchronized cultures of P. Falciparum of the 3D7 strain (the chloroquine-sensitive) and the G-2300 strain (the chloroquine resistant) with the concentrations of extract in the microwells were 100, 10, 1.0, 0.1, and 0.01ug/mL for 48 hours incubation. Chloroquinediphosphates was used for positive control with the concentrations of 10, 1.0, 0.1, 0.01 and 0.001 ug/mL, and DMSO for negative control. The IC50 values found for the methanol extract were 4.230g/mL on the 3D7, and 16.5580 ug/mL on the G-2300 strain. At the same test condition, the chloroquinediphospates has the IC50 of 0.003 ug/mL on the 3D7 strain. From the results of the examination, it can conclude that methanol extract A. champeden is categorized as strong or very active antimalarial against the chloroquine-sensitive P. Falciparum strain, and moderate or active against the chloroquine-resistant strain.

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