Uji Aktivitas Antibakteri Ekstrak Etanol Daun Tumbuhan Sentigi (Pemphis Acidula) Asal Pantai Lalendo Bolok Terhadap Bakteri Pembusuk Ikan Dan Penelusuran Senyawa Aktifnya

RAHMAWATI, Sri (2019) Uji Aktivitas Antibakteri Ekstrak Etanol Daun Tumbuhan Sentigi (Pemphis Acidula) Asal Pantai Lalendo Bolok Terhadap Bakteri Pembusuk Ikan Dan Penelusuran Senyawa Aktifnya. Undergraduate thesis, Universitas Katolik Widya Mandira.

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Abstract

The plant of Sentigi (Pemphis acidula), which grows on the beach of Kupang's Lalendo Bolok, has been used by Bolok people as a traditional medicine for typhus. This study aims to determine and antibacterial activity of sentigi leaf extract to tuna decomposing bacteria, and tracing its active compounds and than to collect natural compounds senti plants from Lalendo Bolok beach as af the natural fish preservatives. A total of 315 grams of powdered dry samples were extracted through the maceration method using 70% ethanol for 12 x 24 hours of 87,96 gram thick extract was obtained with a yield of 27,9%. The physical characteristics of the extract were thick, deep green, not sticky and soluble in distilled water. The phytochemical test was carried out by the Harborn (1984) method and obtained positively containing the active compounds of flavonoids, alkaloids and tannins. The antibacterial activity of the crude extract of the sample was carried out by the method of diffusion wells against bacterial isolates isolated from the outer shell of fish (bacterial isolate A), and which were isolated from fish gills (bacterial B isolate). The test results obtained data that the sample extract had bacteriostatic antibacterial activity against both types of bacterial isolates. The inhibition concentration grows minimum occurs at a concentration of 6.125%, with the area of clear zone for isolates A and B each of 1.50 mm and 1.73 mm. The search for active compounds in sample extracts was carried out through column fractionation using the silica gel stationary phase and eluent in the form of benzene combination: ethyl acetate 5: 1 (v / v). The test 4 groups of fractions. Identification through the UV-Vis spectrophotometer, absorption at wavelength 250 nm and absorbance 2,395 is a transition. Identification through infrared data sample fraction having absorption bands wave area 2950,99 cm-1 is a C-H alkuna functional group, in the wavelength area 1033 it is a O-H functional groups. Where wave number 3400,70 cm-1 is a N-H amin function groups. The functional groups identified are functional groups of Alkaloid and Flavonoid compounds.

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