Skrining Fitokimia dan Karakterisasi Senyawa Metabolit Sekunder Dari Kulit Batang Tumbuhan Niko (Grewia koordersiana Burret)

POTE, Lodowik Landi and NADUT, Anggelinus (2024) Skrining Fitokimia dan Karakterisasi Senyawa Metabolit Sekunder Dari Kulit Batang Tumbuhan Niko (Grewia koordersiana Burret). Skrining Fitokimia dan Karakterisasi Senyawa Metabolit Sekunder Dari Kulit Batang Tumbuhan Niko (Grewia koordersiana Burret), 9 (2). pp. 87-97. ISSN ISSN:261-5081

Text Phytochemical Screening and Characterization of Secondary Metabolite Compounds from the Bark of the Niko Plant (Grewia koordersiana Burret).pdf Restricted to Repository staff only Download (186kB)

Abstract

Phytochemical Screening and characterization of secondary metabolite compounds from the bark of the Niko plant (G. koordersiana Burret) aims to determine the type and characteristics of secondary metabolite compounds.The methods used are extraction, chromatography and spectrophotometry. A total of 500 g of Niko bark powder, macerated with methanol solvent, obtained a thick extract with a yield of 52.09%. The results of phytochemical tests showed that the methanolextract contained alkaloids, flavonoids, terpenoids and saponins. The results of fractionation by column chromatography using methanol eluent: ethyl acetate (1:3) obtained 20 fractions. The results of TLC for each fraction obtained 4 fractions with a single spot, namely fractions 11, 12, 15 and 16, where fractions 11 and 16 had Rf 0.3 and fractions 12 and 15 had Rf 0.7. Fraction A is fraction 11 and 16 with single spot concentrated and obtained extract of 2.09 grams (8.93%) and fraction B is fraction 12 and 15 obtained extract of 2.74 grams (11.53%). The results of characterization by UVVis spectrophotometry for fraction A obtained two absorption bands at wavelengths of 280 and 235 nm. Fraction B obtained two absorption bands at wavelengths of 280 and 245 nm. The results of the characterization by Infrared spectroscopy of methanol extract of Niko bark (G. koordersiana Burret) showed the presence of -OH group at wave number 3383.14 cm-1 and aliphatic C-H group (stretching) at wave number 2983.88 cm-1,carbonyl C=O group at wave number 1741.72 cm-1, aliphatic C-H group (bending) and alcohol C-O group at wavelength 1244.09; 1045.42 and 1033.85 cm-1. Based on the results of the UV-Vis and IR spectra, both isolates contain flavonoids of the flavanone and flavanonol types.

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