Optimasi Pertumbuhan Bakteri Pseudomonas aeruginosa dalam Biodegradasi Hidrokarbon Solar

RUSAE, Maria Filia Secunda (2025) Optimasi Pertumbuhan Bakteri Pseudomonas aeruginosa dalam Biodegradasi Hidrokarbon Solar. Undergraduate thesis, Universitas Katolik Widya Mandira Kupang.

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Abstract

Environmental pollution due to oil spills has become a serious issue threatening the balance of ecosystems and public health, caused by the toxic nature and degradationresistant hydrocarbon compounds. Conventional approaches such as burning and the use of chemical dispersants often result in side effects, hence there is a need for more efficient and environmentally friendly methods. Biodegradation by microorganisms such as Pseudomonas aeruginosa presents a promising alternative due to its ability to break down hydrocarbons through oxygenase and dehydrogenase enzymes. This research aims to investigate the relationship between the number of P. aeruginosa cells and absorbance values, as well as to identify the optimal conditions that support the biodegradation process of oil. The study was conducted experimentally over seven days of incubation with variations in oil concentration (5%, 10%, and 15%), Optical Density (OD: 0.1; 0.5; 1), and NaCl (1%, 3%, and 5%), using Nutrient Broth (NB) and Mineral Salt Medium (MSM). The research results show a linear relationship between the cell count and absorbance values with the regression equation y = 0.1664x + 7.9563 and with the highest absorbance values of 1.123; 1.342; and 1.046 respectively. Conversely, excessively high concentrations can inhibit bacterial growth activity. The optimal condition was obtained at a solar concentration variation of 5% with an absorbance of 1.123, cell count with an OD of 1 with an absorbance value of 1.342, and a NaCl concentration of 1% with an absorbance value of 1.046. In conclusion, P. aeruginosa shows great potential as a bioremediation agent in the effective and sustainable degradation of solar hydrocarbons.

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